The type 4 metallothionein from Brassica napus seeds folds in a metal-dependent fashion and favours zinc over other metals.
Identifieur interne : 000047 ( Main/Exploration ); précédent : 000046; suivant : 000048The type 4 metallothionein from Brassica napus seeds folds in a metal-dependent fashion and favours zinc over other metals.
Auteurs : Agnieszka Mierek-Adamska [Pologne] ; Gra Yna B. D Browska ; Claudia A. BlindauerSource :
- Metallomics : integrated biometal science [ 1756-591X ] ; 2018.
Descripteurs français
- KwdFr :
- Brassica napus (croissance et développement), Brassica napus (génétique), Brassica napus (métabolisme), Cadmium (métabolisme), Conformation des protéines (MeSH), Germination (MeSH), Graines (croissance et développement), Graines (génétique), Graines (métabolisme), Métallothionéine (composition chimique), Métallothionéine (génétique), Métallothionéine (métabolisme), Métaux lourds (métabolisme), Pliage des protéines (MeSH), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Similitude de séquences (MeSH), Séquence d'acides aminés (MeSH), Zinc (métabolisme).
- MESH :
- composition chimique : Métallothionéine, Protéines végétales.
- croissance et développement : Brassica napus, Graines.
- génétique : Brassica napus, Graines, Métallothionéine, Protéines végétales.
- métabolisme : Brassica napus, Cadmium, Graines, Métallothionéine, Métaux lourds, Protéines végétales, Zinc.
- Conformation des protéines, Germination, Pliage des protéines, Similitude de séquences, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Brassica napus (genetics), Brassica napus (growth & development), Brassica napus (metabolism), Cadmium (metabolism), Germination (MeSH), Metallothionein (chemistry), Metallothionein (genetics), Metallothionein (metabolism), Metals, Heavy (metabolism), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Conformation (MeSH), Protein Folding (MeSH), Seeds (genetics), Seeds (growth & development), Seeds (metabolism), Sequence Homology (MeSH), Zinc (metabolism).
- MESH :
- chemical , chemistry : Metallothionein, Plant Proteins.
- chemical , genetics : Metallothionein, Plant Proteins.
- chemical , metabolism : Cadmium, Metallothionein, Metals, Heavy, Plant Proteins, Zinc.
- genetics : Brassica napus, Seeds.
- growth & development : Brassica napus, Seeds.
- metabolism : Brassica napus, Seeds.
- Amino Acid Sequence, Germination, Protein Conformation, Protein Folding, Sequence Homology.
Abstract
The problem of handling zinc in the cell is of great importance because zinc is an indispensable micronutrient involved in most physiological processes in all living organisms. Moreover, our understanding of mechanisms governing the discrimination between micronutrients and toxic metals on the level of individual proteins to the whole-organism level is incomplete. Metallothioneins are able to bind heavy metal ions, and roles in zinc homeostasis have been proposed. Here, we have studied the in vitro and in vivo metal-binding abilities of Brassica napus type 4 metallothionein (BnMT4) and its expression in germinating seeds in response to metal treatment. Our studies on the regulation of MT4 expression by metals at early stages of ontogenic development have revealed for the first time that the mRNA levels of BnMT4 were elevated in response to cadmium and zinc. Given this unexpected metalloregulation, and the dramatic differences in protein folding as detected by 1H NMR spectroscopy, we suggest that the BnMT4 protein may not only have a role in zinc homeostasis in early ontogenesis, but also the potential to discriminate between zinc and cadmium, perhaps via differential recognition of Cd- and Zn-complexes by cellular components involved in protein turnover.
DOI: 10.1039/c8mt00161h
PubMed: 30176031
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Moreover, our understanding of mechanisms governing the discrimination between micronutrients and toxic metals on the level of individual proteins to the whole-organism level is incomplete. Metallothioneins are able to bind heavy metal ions, and roles in zinc homeostasis have been proposed. Here, we have studied the in vitro and in vivo metal-binding abilities of Brassica napus type 4 metallothionein (BnMT4) and its expression in germinating seeds in response to metal treatment. Our studies on the regulation of MT4 expression by metals at early stages of ontogenic development have revealed for the first time that the mRNA levels of BnMT4 were elevated in response to cadmium and zinc. Given this unexpected metalloregulation, and the dramatic differences in protein folding as detected by 1H NMR spectroscopy, we suggest that the BnMT4 protein may not only have a role in zinc homeostasis in early ontogenesis, but also the potential to discriminate between zinc and cadmium, perhaps via differential recognition of Cd- and Zn-complexes by cellular components involved in protein turnover.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30176031</PMID><DateCompleted><Year>2019</Year><Month>09</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1756-591X</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>10</Issue><PubDate><Year>2018</Year><Month>10</Month><Day>17</Day></PubDate></JournalIssue><Title>Metallomics : integrated biometal science</Title><ISOAbbreviation>Metallomics</ISOAbbreviation></Journal><ArticleTitle>The type 4 metallothionein from Brassica napus seeds folds in a metal-dependent fashion and favours zinc over other metals.</ArticleTitle><Pagination><MedlinePgn>1430-1443</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c8mt00161h</ELocationID><Abstract><AbstractText>The problem of handling zinc in the cell is of great importance because zinc is an indispensable micronutrient involved in most physiological processes in all living organisms. Moreover, our understanding of mechanisms governing the discrimination between micronutrients and toxic metals on the level of individual proteins to the whole-organism level is incomplete. Metallothioneins are able to bind heavy metal ions, and roles in zinc homeostasis have been proposed. Here, we have studied the in vitro and in vivo metal-binding abilities of Brassica napus type 4 metallothionein (BnMT4) and its expression in germinating seeds in response to metal treatment. Our studies on the regulation of MT4 expression by metals at early stages of ontogenic development have revealed for the first time that the mRNA levels of BnMT4 were elevated in response to cadmium and zinc. Given this unexpected metalloregulation, and the dramatic differences in protein folding as detected by 1H NMR spectroscopy, we suggest that the BnMT4 protein may not only have a role in zinc homeostasis in early ontogenesis, but also the potential to discriminate between zinc and cadmium, perhaps via differential recognition of Cd- and Zn-complexes by cellular components involved in protein turnover.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mierek-Adamska</LastName><ForeName>Agnieszka</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Genetics, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dąbrowska</LastName><ForeName>Grażyna B</ForeName><Initials>GB</Initials></Author><Author ValidYN="Y"><LastName>Blindauer</LastName><ForeName>Claudia A</ForeName><Initials>CA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Metallomics</MedlineTA><NlmUniqueID>101478346</NlmUniqueID><ISSNLinking>1756-5901</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical><Chemical><RegistryNumber>9038-94-2</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">Metallothionein</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017510" MajorTopicYN="N">Protein Folding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017385" MajorTopicYN="N">Sequence Homology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>9</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30176031</ArticleId><ArticleId IdType="doi">10.1039/c8mt00161h</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pologne</li></country></list><tree><noCountry><name sortKey="Blindauer, Claudia A" sort="Blindauer, Claudia A" uniqKey="Blindauer C" first="Claudia A" last="Blindauer">Claudia A. Blindauer</name><name sortKey="D Browska, Gra Yna B" sort="D Browska, Gra Yna B" uniqKey="D Browska G" first="Gra Yna B" last="D Browska">Gra Yna B. D Browska</name></noCountry><country name="Pologne"><noRegion><name sortKey="Mierek Adamska, Agnieszka" sort="Mierek Adamska, Agnieszka" uniqKey="Mierek Adamska A" first="Agnieszka" last="Mierek-Adamska">Agnieszka Mierek-Adamska</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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